The leaves of some mangroves regulate salt concentrations by accumulating excess salt in special compartments within their cells.

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Mangroves are small shrubs or trees that grow in the presence of salt water along coastlines. Constant exposure to salt water can lead to an imbalance of ions within the plant’s cells, which can have toxic effects on enzymes or interfere with water absorption. To address this, the cells control their internal environment by keeping sodium, potassium, and chloride ions at a stable concentration. Mangroves can survive in especially salty environmental conditions using various strategies that regulate how much salt ends up in their tissues. For example, certain species exclude salt by preventing salt from entering the roots, some excrete excess salt through glands in their leaves, while others accumulate ions in special compartments in their leaves.

Ion sequestration is one mechanism used to relieve the negative effects of salt stress.  Mangroves reduce the amount of sodium ions, for example, in the cell’s main compartment by moving excess sodium into a special membrane-enclosed compartment called a vacuole. By sequestering excess ions into vacuoles, the cell’s main compartment maintains a balanced concentration of ions.

These ions are transported into the vacuole with special proteins embedded in the vacuole membrane, such as proton (hydrogen ion) pumps and sodium-hydrogen antiporters. These membrane proteins work together to build a high concentration of sodium ions in the vacuole. Because the ions are moving from an area of low concentration to a high concentration (against its concentration gradient), this requires energy. First, proton pumps use the energy-rich molecule, ATP, to move protons into the vacuole and establish a proton gradient. This proton gradient functions as stored energy. With the help of the sodium-hydrogen antiporters, the protons flow down their concentration gradient out of the vacuole and into the cell’s main compartment. This releases the stored energy, which is then used to transport sodium ions into the vacuole. The sodium ion concentration in the vacuole builds and the ions remain there until the leaf matures and falls.

This strategy was contributed by Natalie Chen and edited by Dimitri Smirnoff.

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References

“One distinctive structural feature of plant cells is the presence of large membrane-bound compartments, the vacuoles. Early biochemical and tonoplast transport analyses led to the model that the sequestration of excessive Na+ under salt stress is mediated by Na+/ H+ antiporters localized in the vacuolar membrane (Blumwald and Poole, 1985, 1987). These Na+/H+ antiporters mediate Na+ uptake into vacuoles, which is driven by the vacuolar proton gradient established by the vacuolar (V-type) proton ATPase that acidifies the vacuolar lumen (Fig. 1). The resulting vacuolar Na+ sequestration protects essential enzymatic reactions in the cytoplasm from excess Na+ levels while maintaining turgor (Glenn et al., 1999).” (Horie and Schroeder 2004: 2457)

Journal article
Sodium Transporters in Plants. Diverse Genes and Physiological Functions.Plant Physiology, 136: 2456-2462September 1, 2004
Horie T; Schroeder JI

“Vacuolar sequestration of Na+ is an important and cost-effective strategy for osmotic adjustment that also reduces the Na+ concentration in the cytosol. Na+ sequestration into the vacuole depends on expression and activity of Na+/H+ antiporters as well as V-type H+– ATPase and H+– PPase. These phosphatases generate the necessary proton gradient required for activity of Na+/H+ antiporters. Salt accumulation in mangroves occurs with the sequestration of Na+ and Clinto the vacuoles of the hypodermal storage tissue of the leaves (Werner and Stelzer 1990; Aziz and Khan 2001a; Kura-Hotta et al. 2001; Mimura et al. 2003).” (Parida 2010: 203)

Journal article
Salt tolerance mechanisms in mangroves: a reviewTrees, 24(2): 199-217April 1, 2010
Parida AK; Jha B

Journal article
Salt allocation during leaf development and leaf fall in mangrovesTrees, 16: 112-119March 1, 2002
Cram WJ; Torr PG; Rose DA

Journal article
Osmotic adjustment and plant adaptation to environmental changes related to drought and salinityEnvironmental Reviews, 18: 309-319August 20, 2010
Chen H; Jiang JG

Book section
Senescence in mangrovesIn: Sen DN, Rajpurohit KS, editors. Contributions to the ecology of halophytes. London, UK: Dr. W Junk Publishers. p. 173-187.January 1, 1982
Karmarkar SM

Journal article
Rapid increase of vacuolar volume in response to salt stressPlanta, 216: 397-402January 1, 2003
Mimura T; Kura-Hotta M; Tsujimura T; Ohnishi M; Miura M; Okazaki Y; Mimura M; Maeshima M; Washitani-Nemoto S

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