The Dufour’s Gland of bees secretes a multitude of organic compounds like polyesters for nest building, triglycerides for food and hormones for communication.

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Dufour’s Gland is a small gland near the stinger of a bee. It’s an exocrine gland, secreting chemicals, but the purpose of the chemicals and their structure differs from family to family. The secretions can be used for anything from nest building, to reproduction, to pheromones, to the production of larval food. Dufour’s Gland is not unique to bees, being found in many different insects.

Dufour’s Gland can produce a variety of organic compounds, including both saturated and unsaturated hydrocarbons, with long chain hydrocarbons being the most common secretion across bee taxa. More volatile and non-volatile compounds are produced by certain bees, including alcohols, esters, aromatics and fatty acids.

The most common Dufour’s Gland secretion among bees is used to create a waterproof lining for their brood cells, protecting the stashes of food they leave for their larvae, as well as the larvae themselves, from microbes or environmental conditions. The polyester brood cell lining has qualities much like those of cellophane. In the bee genus Colletes, the lining is made up of 18-hydroxyoctadecanoic acid and 20-hydroxy-eicosanoic acid, both stored as lactones in the gland before they are secreted and undergo a chemical reaction to become the lining.

The larvae may also ingest secretions from Dufour’s Gland with their food. In Anthophora abrupta, the secretions are made up of a mixture of liquid triglycerides, one long-chain fatty acid and two short-chain fatty acids before they’re added to the food. 

Dufour’s Gland secretions are also often applied to the entrance of the nest, being unique enough to each individual that they serve as a marker for which nest is their own.

In the Andrena genus the compounds produced by the gland vary based on genetics, implying they may play a role in recognizing kin. In some bees, such as the stingless Meliponini bees, the secretions of a virgin female and a non-virgin female differed by the inclusion of an ester compound, implying they could be used for sexual signalling.

In bumblebees (genus Bombus), Dufour’s Gland increases in size with age and reproductive activity of the Queen, while in workers Dufour’s Gland advertises their sterile status using esters. When an individual develops ovaries, the esters are no longer secreted.

This information is also available from the University of Calgary Invertebrate collection, where it was curated as part of a study on design inspired by bees. 

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“Ground dwelling bees face the challenge of keeping their larval food free from microbial growth, desiccation, and soaking by water. They have evolved to respond to this challenge by coating their brood cell with a hydrophobic lining that forms a sheath around the larval food. Secretions of the Dufour’s gland have been found to be involved in forming this cell lining.” Mitra, 2013. 

“Mated queens had higher proportion of macrocyclic lactones (used in nest construction), and lower proportion of isopentyl esters (volatiles), as compared to virgin queens, and the composition of Dufour’s gland compounds was found to change within one day of mating (Ayasse et al. 1993). In the same species,Lasioglossum malachurum (Halictidae), the pattern of ester composition has also been found to differ between young virgin queens and old nesting queens, and the pattern has also been correlated with volume of fat body and status of ovarian development, implying possible involvement in sex pheromonal communication and in nest recognition”Mitra, 2013. 

Journal article
Function of the Dufour’s gland in solitary and social HymenopteraJournal of Hymenoptera researchMitra A.

Journal article
Natural Polyesters: Dufour's Gland Macrocyclic Lactones Form Brood Cell Laminesters in Colletes BeesScienceHefetz A, Fales HM, Batra SW.

Journal article
Anthophora Bees: Unusual Glycerides from Maternal Dufour's Glands Serve as Larval Food and Cell Lining. ScienceNorden B, Batra SW, Fales HM, Hefetz A, Shaw GJ.

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